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Note: EC stem cells adhere and recover rapidly, forming a fully con uent monolayer in 3 4 days when maintained at 37 C under a humidi ed atmosphere of 5% CO2 in air (see Fig. 6.3). Cells should not require feeding during this time. Passages involving 1:2 to 1:4 split ratios can be used depending on the con uence of the culture before passaging. It is not necessary to wash the cells for passaging after collection using trypsin, provided that suf cient growth medium containing FBS has been added to inactivate trypsin. 6.4. CHARACTERIZATION 6.4.1. Determination of EC Phenotype One of the most straightforward approaches to determining the phenotype of cultured human EC stem cells is to examine the expression of cell surface antigens with ow cytometry. The method is relatively simple and provides accurate, quanti able data regarding the status of the culture and the proportions of stem cell and differentiated cell phenotypes. Comparison of the expression of a variety of antigens on a large panel of human cell lines derived from germ cell tumors con rmed that the cell surface antigens stage-speci c embryonic antigens-3 (SSEA-3) and -4 (SSEA-4) and TRA-160 are particularly characteristic markers of human EC stem cells [Andrews et al., 1996]. In general, cultures of human EC stem cells maintained at high density postcon uence and under optimal growth conditions express high levels of these markers. However, suboptimal culture conditions and differentiation result in the decreased expression of, for example, SSEA-3, and the upregulation of proteins associated with particular pathways of cell differentiation [Andrews, 1982; Przyborski et al., 2000]. Protocol 6.5 provides a general method for the ow cytometric analysis of human EC stem cells.

First, there are three panes in which to view the story: n The Story pane (choose View Story View or press Option+ +G or Ctrl+Alt+G) is the plainest view, essentially the same as the InDesign Story Editor. Here, you work on text as though you were in a word processor, with applied styles shown at left. The left side of Figure 35.2 shows this view. n The Layout pane (choose View Layout View or press +L or Ctrl+L) shows the story as it appears in InDesign. The middle of Figure 35.2 shows this view. n The Galley pane (choose View Galley View or press +G or Ctrl+G) is like the Story pane except that each line breaks as it does in InDesign, so you can see where lines end and whether the last lines in paragraphs have room for more text or whether small text deletions could easily eliminate a short last line during copyfitting.

n For the Select clause of the SQL query, expand the Tables section of the tree to pick the desired table and, under that table, the desired column. With the column selected, click the SELECT button. n You can only add one column at a time; repeat this step to include additional columns. n For the WHERE clause of the SQL query, choose the desired column and click the WHERE button. Complete the clause by entering an operator (such as =, <, >, or LIKE) and a comparative value. The value may be a constant or a variable defined in the Variables area. n For the ORDER BY clause of the SQL query, choose the desired column and click the ORDER BY button. Add the keyword ASC for an ascending sort or DESC for a descending sort.

Using GridPort technologies [3] as described for CEPAR, our efforts began with the creation of a portal for carrying out chemistry computations for understanding various details of structure and property for molecular systems the General Atomic Molecular Electronic Structure Systems (GAMESS) [6] quantum chemistry portal (http://gridport. npaci.edu/gamess). The GAMESS software has been deployed on a variety of computational platforms, including both distributed and shared memory platforms. The job submission page from the GAMESS portal is shown in Figure 40.3. The portal uses Grid technologies such as the SDSC s GridPort toolkit [3], the SDSC SRB [5] and Globus [7] to assemble and monitor jobs, as well as store the results. One goal in the creation of a new architecture is to improve the user experience by streamlining job creation and management. Related molecular sequence, structure, and property software have been created using similar frameworks, including the AMBER [8] classical molecular dynamics portal, the EULER [9] genetic sequencing program, and the Adaptive Poisson-Boltzmann Solver (APBS) [10] program for calculating electrostatic potential surfaces around biomolecules. Each type of molecular computational software provides a level of understanding of molecular structure that can be used for a larger scale understanding of the function. What is needed next are strategies to link the molecular scale technologies through the data and/or through novel new algorithmic strategies. Both involve additional Grid technologies. Development of portal infrastructure has enabled considerable progress towards the integration across scale from molecules to cells, linking the wealth of ligand-based data present in the PDB, and detailed molecular scale quantum chemical structure and

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Holographic Recording Material black or light absorbing. Since an entire crystal will be exposed if only a small part of is hit by photons, it follows that bigger crystals (with more area to catch photons) make for a more light-sensitive emulsion. Emulsions suitable for holography have very small crystals in order to be able to resolve fine fringe patterns and thus aren t very sensitive.

To change several tags on a single song, use the Item Information dialog box like this:

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